yflS [2019-01-04 11:32:28]

malate transporter

locus: BSU07570

pI: 9.74

mw: 51.27 kDa

protein length: 478 aa

gene length: 1434 bp

function: malate uptake

product: malate transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 829,382 → 830,818

RNA

additional information

• the mRNA is stabilized by basepairing with the RoxS RNA [pubmed|28436820]
• non-protected mRNA is subject to degradation by by [SW|rnjA|RNase J1] [pubmed|28436820]

The protein

Catalyzed reaction/ biological activity

• uptake of malate

Expression and Regulation

Operons

• genes: [gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]
  description: [Pubmed|21815947]

  regulatory mechanism

  • [protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR]: activation, in [regulon|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR regulon]

  regulation

  • induced by malate ([protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR])

  additional information

  • the mRNA is substantially stabilized upon depletion of [SW|Rny|RNase Y ][PubMed|21815947]
  • the mRNA is stabilized by basepairing with the [protein|search|RoxS ]RNA [pubmed|28436820]
  • non-protected mRNA is subject to degradation by by [SW|rnjA|RNase J1] [pubmed|28436820]

Biological materials

Mutant

• MGNA-C249 (yflS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2247 NBRP B. subtilis, Japan]
• GP1450 (cat), available in [SW|Jörg Stülke]'s lab
• BKE07570 (Δ[gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG
• BKK07570 (Δ[gene|73356B73D6C89D6569CECCC7295AF85CC7A1E6E3|yflS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG

References

• 12949159,21815947,28436820,29651979