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yflS [2019-01-04 11:32:28]
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yflS [2019-01-04 11:32:28]

malate transporter
Locus
BSU07570
Isoelectric point
9.74
Molecular weight
51.27 kDa
Protein length
478 aa Sequence Blast
Gene length
1434 bp Sequence Blast
Function
malate uptake
Product
malate transporter
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Transporter for organic acids
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    829,382 → 830,818

    RNA

    Additional information

  • the mRNA is stabilized by basepairing with the RoxS RNA PubMed
  • non-protected mRNA is subject to degradation by by RNase J1 PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • uptake of malate

    • Expression and Regulation

    Operons

    Genes
    yflS
    Description
    PubMed

    Regulatory mechanism

  • MalR: activation, in MalR regulon

    • Regulation

  • induced by malate (MalR)

    • Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the mRNA is stabilized by basepairing with the RoxS RNA PubMed
  • non-protected mRNA is subject to degradation by by RNase J1 PubMed
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    Biological materials

    Mutant

  • MGNA-C249 (yflS::erm), available at the NBRP B. subtilis, Japan
  • GP1450 (cat), available in Jörg Stülke's lab
  • BKE07570 (ΔyflS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG
  • BKK07570 (ΔyflS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAATAGGTCTCCTCCTA, downstream forward: _UP4_TAGAAAGAAAAAGGCAGACG

    • References

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